This Pre-made optional inducible lentiviral particles expresses human target, VSIR (V-set immunoregulatory receptor) (alternative name: B7-H5; B7H5; DD1alpha; GI24; PP2135; SISP1; VISTA). The sub-cloned codon sequence is identical (100% match) to CDS region  in NCBI ID: NM_022153.

The full sequence-verified target is natively expressed under an optional inducible suCMV promoter. This lentivirus can be used for regular constitutive high expression (without any induction) or used for inducible expression with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.

A GFP-Puromycin fusion dual marker under the RSV promoter allows the selection of  transduced cells via fluorescent sorting or Puromycin killing.  The GFP signal additionally provides convenient, real-time monitoring of the particles’ performance.

Each Lentivirus is validated on lot-by-lot basis, and target expression is guaranteed.  See Product Manual for details (.pdf).

Amount: 200ul, at 1 x 10⁷ IFU/ml in DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (Note: When specifically requested, the concentrated particles can be provided at 1 x 10⁸ IFU/ml in 200ul PBS solution for $650.00 USD).

Cat#: LVP820-GP