Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheGFPMarkerunderthesameEF1apromoter.APuromycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviaPuromycinantibioticselection.

CREandGFPareconstitutivelyexpressedinhighlevel,indenpendentofcelltypes.GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.  

CREprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforhard-to-transducedcellline,orserum-freecell culture, ordirectlyused forinvivoapplication.

Seeproductmanualfordetails(.pdf).

Amount:  200ul/pervial,at 5x10⁷IFU/mlinPBS. 

Cat#:LVP526-PBS