Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:nuclearpermeableCRE recombinaseandtheGFPMarkerunderthesameEF1apromoter.APuromycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviaPuromycinantibioticselection. CREandGFPareconstitutivelyexpressedinhighlevel,indenpendentofcelltypes.GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance. CREprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites. ParticleswasconcentratedandresUSPendedinPBSsolution,bestsuitableforhard-to-transducedcellline,orserum-freecell culture, ordirectlyused forinvivoapplication. Seeproductmanualfordetails(.pdf). Amount: 200ul/pervial,at 5x107IFU/mlinPBS. Cat#:LVP526-PBS
