PremadeoptionalinducIBLeshRNAlentivirus,knockdownfireflyluciferasegene.ParticlescontainsaGFP-PuromycindualselectionMarker.GFPfluorescentsignalprovidea convenientmethodtoobservethevirustransductionundermicroscope,andthetransducedcellscanbesorted/selectedviaeitherfluorescentsignalorPuromycin.
shRNAsequencewasdesignedin fireflyluciferasecodonregion,demonstratinggreaterthan75%knockdownlevelfor exogenous(trasnfectedluciferaseexpression).ThisistheconcentratedshRNAlentiviurs,providedinPBSsolutionas200ul/vialat5 x10e7 IFU/ml,usedforhard-to-transducedcelltypesorforserum-freecellculture.
Note:Its matchednegativecontrolshRNAlentivirusis:CAT#H1(shRNA-Ctr)-GP-PBS.
Pleaseseeproductmanualformoredetails.
Amount:200ul/tube at(1x107IFU/ml)in PBS
Cat#:LVP345-GP-PBS
