PremadeoptionalinducIBLeshRNAlentivirus,knockdown fireflyluciferasegene.ParticlescontainsaGFP-BlasticidindualselectionMarker.GFPfluorescentsignalprovidea convenientmethodtoobservethevirustransductionundermicroscope,andthetransducedcellscanbesorted/selectedviaeitherfluorescentsignalorBlasticidin.
shRNAsequencewasdesignedin fireflyluciferasecodonregion,demonstratinggreaterthan75%knockdownlevelsforexogenous(trasnfected luciferaseexpression)inHEK293cells. ThisistheconcentratedshRNAlentiviurs,providedinPBSsolutionas200ul/vialat5 x10e7 IFU/ml,usedforhard-to-transducedcelltypesorforserum-freecellculture.
Note:Its matchednegativecontrolshRNAlentivirusis:CAT# H1(shRNA-Ctr)-GB-PBS
Pleaseseeproductmanualformoredetails.
Amount:200ul/tube at(1x107IFU/ml)in PBS
Cat#:LVP345-GB-PBS
