PremadeoptionalinducIBLeshRNAlentivirus,knockdowningβ-Galactosidase(lacZ)gene.ParticlescontainsaRFP-BlasticidindualselectionMarker.RFPfluorescentsignalprovidea convenientmethodtoobservethevirustransductionundermicroscope,andthetransducedcellscanbesorted/selectedviaeitherfluorescentsignalorBlasticidin.
shRNAsequencewasdesignedin β-Galactosidase(lacZ)codonregion,demonstratinggreaterthan90%knockdownlevelsforexogenous(trasnfected lacZexpression). ThisistheconcentratedshRNAlentiviurs,providedinPBSsolutionas200ul/vialat5 x10e7 IFU/ml,usedforhard-to-transducedcelltypesorforserum-freecellculture.
Note:Its matchednegativecontrolshRNAlentivirusis:CAT# H1(shRNA-Ctr)-RB-PBS
Pleaseseeproductmanualformoredetails.
Amount:200ul/tube at(1x107IFU/ml)inPBS,
Cat#:LVP344-RB-PBS
