PremadeoptionalinducIBLelentiviralshRNAelentivirus,knockdowningβ-Galactosidase(lacZ)gene.ParticlescontainsaGFP-BlasticidindualselectionMarker.GFPfluorescentsignalprovidea convenientmethodtoobservethevirustransductionundermicroscope,andthetransducedcellscanbesorted/selectedviaeitherfluorescentsignalorBlasticidin.
shRNAsequencewasdesignedinβ-Galactosidasecodonregion,demonstratinggreaterthan90%knockdownlevelsforexogenous(trasnfected lacZexpression)inHEK293cells.ThisistheconcentratedshRNAlentiviurs,providedinPBSsolutionas200ul/vialat5 x10e7 IFU/ml,usedforhard-to-transducedcelltypesorforserum-freecellculture.
Note:Its matchednegativecontrolshRNAlentivirusis:CAT# H1(shRNA-Ctr)-GB-PBS
Pleaseseeproductmanualformoredetails.
Amount:200ul/tube at(1x107IFU/ml)in PBS
Cat#:LVP344-GB-PBS
