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Gentarget/shRNA (h P53)-(RFP-Bsd) lentivirus in PBS/LVP343-RB-PBS/1 Ea
PremadeshRNAlentivirus,knockdown humanP53gene(NM_000546).ParticlescontainsaRFP-BlasticidindualselectionMarker.RFPfluorescentsignalprovidea convenientmethodtoobservethevirustransductionundermicroscope,andthetransducedcellscanbesorted/selectedviaeitherfluorescentsignalorBlasticidin.
shRNAsequencewasdesignedinhumanP53codon(ORF)region,demonstratinggreaterthan75%knockdownlevelsforbothexogenous(trasnfectedP53expression)andendogenousP53expression. ThisistheconcentratedshRNAlentiviurs,providedinPBSsolutionas200ul/vialat5 x10e7 IFU/ml,usedforhard-to-transducedcelltypesorforserum-freecellculture.
Thisparticles isprovidedinDMEMmediumwith10%FBSand10xPolybrene,as200ul/eachat1×10e7IFU/ml.
Note:Its matchednegativecontrolshRNAlentivirusis:CAT# H1(shRNA-Ctr)-RB-PBS
Pleaseseeproductmanualformoredetails.
CAT#: LVP343-RB-PBS
