PremadeoptionalinducIBLeshRNA lentivirus,knockdowninghumanP53gene(NM_000546).ParticlescontainsaGFP-BlasticidindualselectionMarker.GFPfluorescentsignalprovidea convenientmethodtoobservethevirustransductionundermicroscope,andthetransducedcellscanbesorted/selectedviaeitherfluorescentsignalorBlasticidin.
shRNAsequencewasdesignedinhumanP53codon(ORF)region,demonstratinggreaterthan75%knockdownlevelsforbothexogenous(trasnfectedP53expression)andendogenousP53expression. ThisistheconcentratedshRNAlentiviurs,providedinPBSsolutionas200ul/vialat5 x10e7 IFU/ml,usedforhard-to-transducedcelltypesorforserum-freecellculture.
Note:Its matchednegativecontrolshRNAlentivirusis:CAT# H1(shRNA-Ctr)-GB-PBS
Pleaseseeproductmanualformoredetails.
Amount:200ul/tube at(1x107IFU/ml)inPBS
CAT#:LVP343-GB-PBS
