ThreeshRNAlentiviectors(5.0ug/each)andthreepre-madeshRNAexpressionlentivirus(0.5ml/each).OnenegativecontrolshRNAvector(5.0ug)andvirus(0.5ml).Guaranteedknockdown(atleastoneshRNAhas>75%knockdownlevel).
TheshRNAwasexpressedunderthehuman U6constitutivepromoter. TheshRNAlentivectoraslocontainsaPuromycin MarkerunderRsvpromoter,whichallowstoselecttheshRNApositivecellbyPuromycinantibioticselection.SeeshRNAcloningkitmanualformoredetailsabouttheshRNAlentivector
Orderwillbedeliveredwithin7-10businessdays.
Cat#:U6-shRNA(Puro)-Custom,
Amounts:(1)PlasmidDNAs:5ug/eachx4tubes;(2):pre-madeshRNAvirus:0.5ml/eachx4tubes.(vectorsandviruses:threetargetspecificshRNAand onenegativecontrolshRNA).
Note:pleaseprovidethetargetaccessionnumberduringcheckoutinthe“Comments”field,oremailusyourtargetsequencethatyouwanttheshRNAdesignedupon.
