ThreeshRNAlentiviectors(5.0ug/each)andthreepre-madeshRNAexpressionlentivirus(0.5ml/each).OnenegativecontrolshRNAvector(5.0ug)andvirus(0.5ml).
TheshRNAwasexpressedunderanoptionalTetracyclineinducIBLehumanH1promoter.ItcanbeusedasconstitutiveexpressionofshRNAwithoutanyinduction, orusedastetracyclineinducibleexpressionwhentheTet-repressorproteinispresentinadvance.TheshRNAlentivectoraslocontainsaRFP-PuromycinfusiondualMarkerunderRsvpromoter,whichallowstoselecttheshRNApositivecellbyPuromycinantibioticselectionorbyFACScellsortingviaRFPsignal.SeeshRNAcloningkitmanualformoredetailsabouttheshRNAlentivector.
Orderwillbedeliveredwithin7-10businessdays.
Cat#:H1-shRNA(RP)-Custom,
Amounts:(1)PlasmidDNAs:5ug/eachx4tubes;(2):pre-madeshRNAvirus:0.5ml/eachx4tubes.(vectorsandviruses:threetargetspecificshRNAand onenegativecontrolshRNA).
Note:pleaseprovidethetargetaccessionnumberduringcheckoutinthe“Comments”field.
