ThreeshRNAlentiviectors(5.0ug/each)andthreepre-madeshRNAexpressionlentivirus(0.5ml/each).OnenegativecontrolshRNAvector(5.0ug)andvirus(0.5ml).Guaranteedknockdown(atleastoneshRNAhas>75%knockdownlevel).

TheshRNAwasexpressedunderanoptionalTetracyclineinducIBLehumanH1promoter.ItcanbeusedasconstitutiveexpressionofshRNAwithoutanyinduction, orusedastetracyclineinducibleexpressionwhentheTet-repressorproteinispresentinadvance.TheshRNAlentivectoraslocontainsaPuromycin MarkerunderRsvpromoter,whichallowstoselecttheshRNApositivecellbyPuromycinantibioticselection.SeeshRNAcloningkitmanualformoredetailsabouttheshRNAlentivector

Orderwillbedeliveredwithin7-10businessdays.

Cat#:H1-shRNA(Puro)-Custom,

Amounts:(1)PlasmidDNAs:5ug/eachx4tubes;(2):pre-madeshRNAvirus:0.5ml/eachx4tubes.(vectorsandviruses:threetargetspecificshRNAand onenegativecontrolshRNA).

Note:pleaseprovidethetargetaccessionnumberduringcheckoutinthe“Comments”field,oremailusyourtargetsequencethatyouwanttheshRNAdesignedupon.