Pre-madeLentiviralparticles expressing nuclearpermeableCRE recombinase underan optionalinducIBLe suCMV promoter.A RFP Markerwas co-expressedunderthesamepromoter.CRE andRFPwere bicistronicallyexpressed asindividualproteinmediatedbya2Aelement. 

CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ANeomycinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviaNeomycin antibioticselection.

NLS-CREandRFPare constitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducibleexpression asdesired.RFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.  

Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

See ProductManual fordetails(.pdf).

Particleswasconcentratedviaourproprietaryprocedures,andre-sUSPendedinPBSbufferforultrahightitervirus,suitableforalltypesof invivo applications.

Amount: 50ul/pervial,at1x10e9 IFU/mlinPBS. 

Cat#:ULVP-027