Pre-madeLentiviralparticles expressing nuclearpermeableCRE recombinase underan optionalinducIBLe suCMV promoter.A GFP Markerwas co-expressedunderthesamepromoter.CRE andGFPwere bicistronicallyexpressed asindividualproteinmediatedbya2Aelement. 

CREcontainsaNLS(nuclearlocalizationsignal) atitsN-term.This nuclearpermeant CRE increasestherecombinationeventsinvivo.ABlasticidinmarkerwasexpressedunderRSVpromoter.ParticlesallowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviablasticidinantibioticselection.

NLS-CREandGFPare constitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducibleexpression asdesired.GFPsignalprovidesaconvenient,realtimemonitoringthe particlesperformance.  

Creprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

See ProductManual fordetails(.pdf).

Particleswasconcentratedviaourproprietaryprocedures,andre-sUSPendedinPBSbufferforultrahightitervirus,suitableforalltypesof invivo applications.

Amount: 50ul/pervial,at1x10e9 IFU/mlinPBS. 

Cat#:ULVP-337