TransformedfromHelacells vialentiviralsystem,expressingthertTA(reversetetracyclineTranscriptionalActivator)protein. rtTA-m2(ABC65845.1)isconstitutively expressedundertheenhancedEF1apromoter.AGFP-Puromycin fusiondualMarkerwasexpressedunderRSVpromoter(seetheintegratedexpressioncassettebelow).Click ProductManual formoredetails.
ThiscelllineexpressedrtTAproteinbindstotetracyclineoperatorelements(TetO)inthepresenceofdoxycycline(Dox).AnypromotersthatembeddedwithTetOsequencescanbindstothertTAproteinwhentheinducer(Doxortracycline)ispresent,whichactivatesthetranscriptionofthepromoter’sdownstreamgene(Socalled,Tet-OninducIBLesystem).Therefore,thiscelllinecanbeusedforTetracyclineorDoxinducibletargetexpressionwhenappliedwithtargetexpressionvectors(withTetOninduciblepromoter),orforgenerationofanyinducibletargetstablecelllines(afterintroducedaTetOnexpressionvector).
Soldat: 1vialx(>2 x106cells)/vial
Cat#SC038-GP
