Helacellwasderivedfromcervicalcancercells,andwasfoundtoberemarkablydurableandprolificwhichwarrantsitsextensiveuseinscientificresearch.
ThisCas9expressionstablecelllinewastransformedfrom HelacelllineinwhichtheCas9enzymeisdrivenbyourengineeredsuperstrongCMVpromoter(suCMV).TheBlasticidin antibioticselectionwasdrivenundertheRSVpromoter.seetheexpressioncassettebelowthatintegratedintocellline’sgenome.
This CRISPRready(Cas9expression)stablecelllinesmakethegenomiceditingeasierthaneverinHela cells.YousimplydeliverthetargetspecificgRNAintotheCRISPRreadycelllineandselecttheknock-outorknock-incellsforyourdesiredtarget.Noneedtodocas9containingCRISPRcloning,andnoworryaboutthehard-to-deliveredCas9constructs.Youonlyneedtogeneratethemucheasierincloning,andmuchsmallerinsizeofthetarget-gRNAconstructs.Gentargetalsoprovideservicestogenerateyourtargetspecific,ready-to-usegRNAlentivirus.PleasecontactusifyouneedtheCRISPRgRNAlentivirusservices.
Soldat: 1vialx(2 x106cells)/vial
ProductManual.
Cat#SC045-Cas9-Bsd
