TransformedfromHEK293cellsbylentiviralsystemwith sequenceverified humanCFTRgene(NM_000492). CFTR was constitutivelyexpressed underanenhancedEF1a promoter. TheRFP-BlasticidinMarkerwasexpressedunderRsvpromoter.ThecellconstitutivelyexpressbothCFTRandRFP. ThisCFTRproteininvolvedinmulti-drugresistance.ItbelongstoaATP-bindingcassette(ABC)subfamily.TheABCproteinstransportvariousmoleculesacrossextra-andintra-cellularmembranes.TheCFTRfunctionsasachloridechannelandcontrolstheregulationofothertransportpathways.Itischaracterizedinchronicbronchopulmonarydisease(withrecurrentrespiratoryinfections),pancreaticinsufficiency(whichleadstomalabsorptionandgrowthretardation)andelevatedsweatelectrolytes.Mutationsinthisgeneareassociatedwiththeautosomalrecessivedisorderscysticfibrosisandcongenitalbilateralaplasiaofthevasdeferens. Pleaseseedetails in ProductManual. Soldat: 1vialx(2 x106cells)/vial Cat#SC022-RB
