Transformedfrommouseadhesive CT6 cellline(anN-nitroso-N-methylurethane-(NNMU)induced,undifferentiatedcoloncarcinomacellline)express tetracyclinerepresser(TetR)gene. TetRwasexpressedunderenhancedEF1a promoter.ABlasticidin antibioticMarkerwasexpressedunderRSVpromoter. The followingexpressioncassettewasinsertedintocellline’sgenome.
Itisusedtogenerate tetracyclineinducIBLetargetexpressioncelllinebysimplyinfectthiscelllinewithInducibleexpression particles.Thetargetexpressionthenwillbesilencedinthiscellline,andexpressionwillbeinduceduponadditionofTetracycline. see ProductManual fordetails.
SeeHowinducibleexpressionworks.
Completedculturemedium:
RPMI1640withHEPES/10%FBS/Pen-Strep, optionallyadd:10ug/mlfinalBlasticidin.
Soldat: 1vialx(>2 x106cells)/vial
Cat#SC056-TetR
