ThisA549/Cas9 StableCellLineistransformedfromtheA549humanlungcancer cellline,stablyexpressingthenuclearpenetratinghumanizedwild-typeCas9endonuclease.TheCas9enzymeisdrivenbyourengineeredsuperstrongCMVpromoter(suCMV).It alsocontainstheGFP-Puromycin fluorescent-antibioticfusiondualMarker undertheRSVpromoter. EachcellexpresseshighlevelofCas9enzymeandalsodemonstratestheGFPfluorescentsignalunderfluorescent-microscope.seetheexpressioncassettebelowthatintegratedintocellline’sgenome.
This CRISPRready(Cas9expression)stablecelllinesmakethegenomiceditingeasierthaneverinA549cells.YousimplydeliverthetargetspecificgRNAintotheCRISPRreadycelllineandselecttheknock-outorknock-incellsforyourdesiredtarget.Noneedtodocas9containingCRISPRcloning,andnoworryaboutthehard-to-deliveredCas9constructs.Youonlyneedtogeneratethemucheasierincloning,andmuchsmallerinsizeofthetarget-gRNAconstructs.Gentargetalsoprovideservicestogenerateyourtargetspecific,ready-to-usegRNAlentivirus.PleasecontactusifyouneedtheCRISPRgRNAlentivirusservices.
Soldat: 1vialx(2 x106cells)/vial
ProductManual.
Cat#SC043-Cas9-GP
