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Gentarget/YFP (RFP-Bsd) inducible control lentivirus in PBS/LVP357-PBS/1 Ea
Thisparticleswasproducedinour tetrayclineinducIBLelentiviralsystem.ItexpressescodonoptimizedYFPundertetracyclineinducible suCMVpromoter.ARFP-BlasticidinfusionMarkerunder theconstitutiveRSVpromoter,allowstoselecttransducedcellsviablasticidinantibioticorRFPsignalsorting.
YFP canbe constitutivelyexpressedinhighlevelwithoutanyinduction.Butoptionally, thisparticles canalsobe usedasTetracyclineinducibleexpressionofYFPintheprescenceofTetRprotein(repressor).Soitcanbeusedalonefordoublefluorescentexpression (YFPandRFP)particles,orusedasthecontrolparticlestovalidateyourtetracylcineinduciblesystemwhenTetRispresent.Pleaseseeproductmanualfordetails.
BothYFPandRFPsignal canbevisulizedunderfluorescentmicrosope.TheRFPisconstitutivelyexpressedandnotaffectedbyTetracycline inducation.
The particleswasconcentratedandbufferexchangedintoPBS.(See productmanual (.pdf).
Amount: 200ul/pervial,at1x107IFU/mlinPBS.
Cat#:LVP357-PBS
