Thisparticleswasproducedinour tetrayclineinducIBLelentiviralsystem.ItexpressescodonoptimizedRFPundertetracyclineinducible suCMVpromoter.AGFP-PuromycinfusionMarkerunder theconstitutiveRSVpromoter,allowstoselecttransducedcellsviaPuromycinantibioticorGFPsignalsorting.
RFP canbe constitutivelyexpressedinhighlevelwithoutanyinduction.Butoptionally, thisparticles canalsobe usedasTetracyclineinducibleexpressionofRFPintheprescenceofTetRprotein(repressor).Soitcanbeusedalonefordoublefluorescentexpression (RFPandGFP)particles,orusedasthecontrolparticlestovalidateyourtetracylcineinduciblesystemwhenTetRispresent.PleaseseeProductManualfordetails.
BothRFPandGFPsignal canbevisulizedunderfluorescentmicrosope.TheGFPisconstitutivelyexpressedandnotaffectedbyTetracycline inducation.
The particleswasconcentratedandbufferexchangedintoPBS.
Amount: 200ul/pervial,at 5x107IFU/mlinPBS.
Cat#:LVP531-PBS
