Thisparticleswasproducedinour optional inducIBLelentiviralsystem.ItexpressescodonoptimizedRFPundermodifiedTetCMVpromoter.AGFP-PuromycinfusionMarkerunder theconstitutiveRSVpromoter,allowstoselecttransducedcellsviaPuromycinantibioticorGFPsignalsorting.
RFP canbe constitutivelyexpressedinhighlevelwithoutanyinduction.Butoptionally, thisparticles canalsobe usedasTetracyclineinducibleexpressionofRFPintheprescenceofTetRprotein(repressor).Soitcanbeusedalonefordoublefluorescentexpression (RFPandGFP)particles,orusedasthecontrolparticlestovalidateyourtetracylcineinduciblesystemwhenTetRispresent.PleaseseeProductManual fordetails.
BothRFPandGFPsignal canbevisulizedunderfluorescentmicrosope.TheGFPisconstitutivelyexpressedandnotaffectedbyTetracycline inducation.
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBSwith10xpolyberne.
Cat#:LVP531
