Thisparticleswasproducedinour tetrayclineinducIBLelentiviralsystem.ItexpressescodonoptimizedGFPundertetracyclineinducible TetCMVpromoter.ARFP-BlasticidinfusionMarkerunder theconstitutiveRSVpromoter,allowstoselecttransducedcellsviablasticidinantibioticorRFPsignalsorting.
GFPisconstitutivelyexpressedinhighlevelwithoutanyinduction.Butoptionally, thisparticles canbe usedasTetracyclineinducibleexpressionofGFPintheprescenceofTetRprotein(repressor).Soitcanbeusedalonefordoublefluorescentexpression (GFPandRFP)particles,orusedasthecontrolparticlestovalidateyourtetracylcineinduciblesystemwhenTetRispresent.
BothGFPandRFPsignal canbevisualizedunderfluorescentmicrosope.TheRFPisconstitutivelyexpressedandnotaffectedbyTetracycline induction.
The particleswasconcentratedandbufferexchangedintoPBS.(Pleaseseeproductmanualfordetails.
Amount: 200ul/pervial,at 5x107IFU/mlinPBS.
Cat#:LVP024-PBS
