Thisparticleswasproducedinour tetrayclineinducIBLelentiviralsystem.ItexpressescodonoptimizedGFPundertetracyclineinducible suCMVpromoter.ARFP-BlasticidinfusionMarkerunder theconstitutiveRSVpromoter,allowstoselecttransducedcellsviablasticidinantibioticorRFPsignalsorting.
GFPisconstitutivelyexpressedinhighlevelwithoutanyinduction.Butoptionally, thisparticles canbe usedasTetracyclineinducibleexpressionofGFPintheprescenceofTetRprotein(repressor).Soitcanbeusedalonefordoublefluorescentexpression (GFPandRFP)particles,orusedasthecontrolparticlestovalidateyourtetracylcineinduciblesystemwhenTetRispresent.PleaseseeProductmanualfordetails.
BothGFPandRFPsignal canbevisualizedunderfluorescentmicroscope.TheRFPisconstitutivelyexpressedandnotaffectedbyTetracycline induction.
Amount: 200ul/pervial,at1x107IFU/mlinDMEMmediumwith10%FBSwith10xpolyberne.
Cat#:LVP024