Pre-madelentivirusexpress reversetetracyclineTranscriptionalActivator(rtTA)protein.Lentiviralparticles expressingrtTA-m2 gene(ABC65845.1) underanenhancedEF1apromoter(whichisnon-tissue,non-celltypespecificpromoter,notbesilenced afterlong-termculture). AGFP-Puromycin fusiondualMarkerunder RSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaGFPsignalorviaPuromycin antibiotic(Dualmarkers).SeeProductManual(.pdf).
rtTA-M2isamutantofrtTAthathasincreasedstABIlity,reducedbackgroundexpressionintheabsenceofDoxycycline(Dox,tetracyclineanalog)andimprovedinducibilityinthepresenceofDox.ItisusedforTetOninducIBLeexpressionsystem.
ThisistheGFP-Puromycindualmarker versionofrtTAparticlesinDMEMmediumwith10%FBSwith10%FBSand10xPolybrene.
Amount: 200ul/pervial,at1x107IFU/ml
Cat#:LVP400-GP
