Pre-madelentivirusexpress reversetetracyclineTranscriptionalActivator(rtTA) protein.Lentiviralparticles expressing rtTA-m2 gene(ABC65845.1) under anenhancedEF1apromoter (whichisnon-tissue,non-celltypespecificpromoter,notbesilenced afterlong-termculture). A GFP-blastidicin fusiondualMarkerunder RSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionvia GFP signalorvia blasticidin antibiotic(Dualmarkers).See ProductManual (.pdf).
rtTA-M2 isamutantofrtTAthathasincreasedstABIlity,reducedbackgroundexpressionintheabsenceofDoxycycline(Dox,tetracyclineanalog)andimprovedinducibilityinthepresenceofDox.Itisusedfor TetOn inducIBLeexpressionsystem.
ThisistheGFP-Blasticidindualmarker versionofrtTAparticles,concentratedandprovidedinPBS.
Amount: 200ul/pervial,at5x107IFU/ml.
Cat#:LVP400-GB-PBS
