Pre-madelentiviralparticlesexpressingafusiontargetof(RFP-Annexin5). ThesequenceverifiedfulllengthumanAnnexin5-ORF(NCBIaccesionID:NM_001154 ) wasfusioned inframewithRFPatitsN-terminal,expressedunderaconsitutivesuCMVpromoter, containingapuromycin antibiotic Markerunderthe RSVpromoter.
FluorescentlabelledAnnexin5(or AnnexinV)canbeusedtodetectearlyapoptoticcells.Annexin5interactsstronglyandspecificallywithphosphatidylserine(PS)thatexposuredfromplasmamembraneintheearlyeventinapoptosis.Therefore,Annexin5canbeusedtodetectapoptosisbytargetingforthelossofplasmamembraneasymmetry.LabeledannexinVcanbeappliedbothinflowcytometryandinlightmicroscopyinlivingcultureculture.
Pleaseseeproductmanualfordetails.
Amount:200ul/vial(1×108IFU/ml), providedinDMEMmediumwith10%FBSand60ug/mlPolybrene(10x).
Cat#:LVP445-R
