Pre-madeinducIBLelentiviralparticlesforexpressing mousetarget: Sox2(alternativename:lcc;ysb;Sox-2),100%matchedCDSregioninNCBIID:NM_011443.3.

FullsequenceverifiedtargetisnativelyexpressedunderatetracyclineinduciblesuCMVpromoter.ARFP-blasticidinfusionMarkerunderRSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibiotic(Dualmarkers).RFPsignalprovidesaconvenient,realtimemonitoringtheparticlesperformance.

Particlescanbeused forconstitutivehighexpressionformakingmouseiPScells.Ifinducibleexpressinisdesirable,itcanbeusedastetracyclineinducibleexpressiononlywhenarepressorregulatorprotein(tetR)ispresentinadvance(seeinduciblelentiviralsystemfordetails).Eachparticleswasvalidatedinlotbylotbasisandtargetexpressionisguaranteed.Particleswerepackagedinserum-freesolutioncontainingnoanyhumanoranimalorigincomponents.SeeProductManual fordetails(.pdf).

Amount:200ul/tube, at1x10e7 IFU/mlinDMEMmediumwith10%FBS. 

Cat#:LVP004m