Pre-madeinducIBLelentiviralparticlesforexpressing mousetarget: NANOG(alternativename:ENK,ecat4),100%matchedCDSregioninNCBIID:NM_028016.2.
FullsequenceverifiedtargetisnativelyexpressedunderatetracyclineinduciblesuCMVpromoter.ARFP-blasticidinfusionMarkerunderRSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibiotic(Dualmarkers).RFPsignalprovidesaconvenient,realtimemonitoringtheparticlesperformance.
Particlescanbeused forconstitutivehighexpressionformakingmouseiPScells.Ifinducibleexpressinisdesirable,itcanbeusedastetracyclineinducibleexpressiononlywhenarepressorregulatorprotein(tetR)ispresentinadvance(seeinduciblelentiviralsystemfordetails).Eachparticleswasvalidatedinlotbylotbasisandtargetexpressionisguaranteed.Particleswerepackagedinserum-freesolutioncontainingnoanyhumanoranimalorigincomponents.Seeproductmanualfordetails(.pdf).
Amount:200ul/tube, at1x108IFU/mlinserumfreemedium.
Cat#:LVP005m
