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Gentarget/m DLL (GFP-Puro) inducible lentiviral particles/LVP908-GP/1 Ea
ThisPre-madelentivirusexpressesaCDNAclone, mouse target: DLL1[mousedelta-like1/Drosophila)]. (alternativename:Delta1).Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID: NM_007865
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducIBLe suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction),oroptionallyusedfor inducibleexpression by tetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent(PremadeTetRlentivirus canbepurchasedseparatelyifyouneedtheinducibleexpression).
Thelentiviruscontainsa GFP_Puromycin fusiondualMarkerundertheRSVpromoterwhichallowsfluorescencesortingorblasticidinkilling selectionof transducedcells. TheRFPsignalprovidesconvenient,real-timemonitoringofthevirus’ performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManual fordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP908-GP
