ThisPre-madeoptionalinducIBLelentiviralparticles expressesmouse target,CXCR5:chemokine(C-X-Cmotif)receptor5 (alternativename:Blr1;CXC-R5;CXCR-5;Gpcr6;MDR15 ).Thesequenceis100%matchedCDSregion (NCBIID:NM_007551 ).
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpressionorinducibleexpressionwithtetracyclineorDoxwhenthetetracyclinerepressorprotein(TetR)ispresent.SeeProductManualfordetails.A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene.(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionforhigherprice).
Cat#:LVP618
