Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:fireflyluciferaseandnuclearpermeableCRE recombinase,underthesamesuCMVpromoter.ARFP-BlasticidinfusiondualMarkerwasexpressedunderRSVpromoter,whichallowtoselectthepositivetransducedstablecellsforlongtermcultureviaRFPcellsortingorviaantibioticselection.
BothlucifeaseandCREarehighlyactive,bestforbothenzymes’assaysInvivoorInvitro.CREprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.This nuclearpermeant CRE(NLS-CRE) increasestherecombinationeventsinvivo. PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.
LuciferaeandNLS-CRE areconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired.LuciferaseandRFPprovide theconvenient,realtimemonitoring signals.
ThisparticlesisconcentratedandbufferchangedintoPBSsolution,bestsuitableforhard-to-transducedcelllines,orserum-sensitivecelllinesandinvivoapplication.
Seeproductmanualfordetails(.pdf).
Amount: 200ul/pervial,at 5x107IFU/mlinPBS.
Cat#:LVP413-PBS
