Pre-madelentiviralparticlesbicistronicallyexpresstwoindividualproteins:fireflyluciferaseandnuclearpermeableCRE recombinase,underthesamesuCMVpromoter.AGFP-BlasticidinfusiondualarkerwasexpressedunderRSVpromoter,whichallowtoselectthepositivetransducedstablecellsforlongtermcultureviaGFPcellsortingorviaantibioticselection.

BothlucifeaseandCREarehighlyactive,bestforbothenzymes'assaysInvivoorInvitro.CREprotein,frombacteriophageP1,catalyzesrecombinationbetween34basepairtargetsequences, namedloxsites.CRElentiviralparticles can catalyze thejointingeventInVivo inthepresenceof loxsites.This nuclearpermeant CRE(NLS-CRE) increasestherecombinationeventsinvivo. PurifiedCREenzymecanjointindividualplasmidscontainingloxsites.

LuciferaeandNLS-CRE areconstitutivelyexpressedinhighlevel,andoptionally,alsocanbeusedastetracyclineinducIBLeexpression asdesired. LuciferaseandGFPprovide theconvenient,realtimemonitoring signals.  

Seeproductmanualfordetails(.pdf).

Amount:  200ul/pervial,at 1x10⁷IFU/mlinDMEMmediumwith10%FBS. 

Cat#:LVP411