Thepre-madelentivirusconsitutivelyexpressesanuclearpermeablelight-activatorproteinunderstrongCMVpromoter.It isaphotosensitiveproteinderivedfromErythrobacterlitoralisproteinEL222.Itconsistsoftwodomains,thelightsensordomain(LSDorcalledLOV:light-oxygen-voltage)andtheDNAbindingdomain(DBDorcalledHTH:helix-turn-helix).Inthedark,thetwodomainareinteractedwitheachothertopreventitsbindingto“lightinducIBLepromotersequence”.Whenbluelightison(465nm),theIlluminationbreakstheseinteractions(proteinformationchanges),openingtheDBDdomainfromLSDdomain,whichtriggerstheDBDbindingtothelight-induciblepromotersequence,andturnonthedownstreamreportexpression.

Thisproduct containsapuromycin selectionMarkerunderRSVpromoter.Clickto See ProductManual fordetails(.pdf).

Amount:200ul/pervialinDEMEmedium/10%FBS/10xPolybrene

CAT#:LVP473