ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,TSPO (translocatorprotein(18kDa),transcriptvariantPBR) (alternativename:BPBS;BZRP;DBI;IBP;MBR;mDRC;PBR;PBS;pk18;PKBS;PTBR). Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID:NM-000714.
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction)orusedfor inducibleexpression withtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.
A GFP-Puromycin fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheGFP signaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.(SeealsotheRFP-Blasticidinversionofthisproduct,CAT#LLVP834).
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP834-GP
