Pre-madeoptionalinducIBLelentiviralparticlesforexpressinghumantarget:TRPV1(alternativename:VR1,OTRPC1).Thesub-clonedcodonsequenceisidentical(100%match)toCDSregioninNCBIID:NM_080704.3 .ParticlesalsocontainsaRFP-BlasticidindualselectionMarker.
Full sequenceverifiedtargetisnativelyexpressedunderatetracyclineinduciblesuCMVpromoter(Note:itcan alsousedasregularconstitutivehighexpressionwithoutanyinducationrequired).ARFP-BlasticidinfusionmarkerunderRSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibiotic(Dualmarkers).RFPsignalprovidesaconvenient,realtimemonitoringtheparticlesperformance.
Eachparticleswasvalidatedinlotbylotbasisandtargetexpressionisguaranteed.Particleswerepackagedinserum-freesolutioncontainingnoanyhumanoranimalorigincomponents.SeeProductManual fordetails(.pdf).
Amount:200ul,at1x107IFU/mlinDMEM medium(with10xpolybrene).
Cat#:LVP503
