ThisPre-madelentivirusexpressesaCDNAclone, human target: TNIP1 (TNFAIP3interactingprotein1).(AlternativeName:ABIN-1;NAF1;nip40-1;VAN).Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID: NM_001252385.1
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducIBLe suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction),oroptionallyusedfor inducibleexpression by tetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent(PremadeTetRlentivirus canbepurchasedseparatelyifyouneedtheinducibleexpression).
Thelentiviruscontainsa Blasticidin-RFP fusiondualMarkerundertheRSVpromoterwhichallowsfluorescencesortingorblasticidinkilling selectionof transducedcells. TheRFPsignalprovidesconvenient,real-timemonitoringofthevirus’ performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManual fordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP900
