ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,TNFSF10: tumornecrosisfactor(ligand)superfamily,member10 (alternativename:Apo-2L;APO2L;CD253;TL2;TRAIL ).Thesequenceis100%matchedCDSregion (NCBIID:NM_003810).
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpressionorinducibleexpressionwithtetracyclineorDoxwhenthetetracyclinerepressorprotein(TetR)ispresent.SeeProductManualfordetails.A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene.(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionforhigherprice).
Cat#:LVP609
