Pre-madeinducIBLelentiviralparticlesforexpressinghumantarget,telomerasereversetranscriptase:TERT(alternativename:EST2,TCS1,TP2,TRT,hEST2),100%matchedCDSregioninNCBIID:NM_198253.2.
Thefull sequence-verifiedtargetisnativelyexpressedunder anoptionalinducible suCMVpromoter. Thislentiviruscan be usedforregularconstitutivehighexpression(withoutanyinduction)orusedfor inducibleexpression withtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.
AGFP-Puromycin fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorPuromycinresistanceselectionof transducedcells. TheGFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. See ProductManual fordetails(.pdf).
Amount:200ul,at1x107 IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108 IFU/mlin200ul PBS solutionfor$650.00USD).
Cat#:LVP105-GP
