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Gentarget/h TDP2 (HA) inducible lentiviral particles/LVP601/1 Ea
ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,TDP2: tyrosyl-DNAphosphodiesterase2 (alternativename:AD022;dJ30M3.3;EAP2;EAPII;hTDP2;TTRAP).Thesequenceis100%matchedCDSregion (NCBIID:NM_016614.2).
Thefull sequence-verifiedtargetisexpressedwithC-terminalHAtagunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpressionorinducibleexpressionwithtetracyclineorDoxwhenthetetracyclinerepressorprotein(TetR)ispresent.SeeProductManualfordetails.A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene.(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionforhigherprice).
Cat#:LVP601