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Gentarget/h TAOK2 (Myc) inducible lentiviral particles/LVP1059/1 Ea
ThisPre-madelentivirusexpressesaCDNAclone, human target: TAOK2 ( TAOkinase2). (alternativename:MAP3K17;PSK;PSK1;PSK1-BETA;TAO1;TAO2).ItisexpressedwiththeMyc tagatitsN-terminal.Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID: NM_016151.3
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducIBLe suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction),oroptionallyusedfor inducibleexpression by tetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent(PremadeTetRlentivirus canbepurchasedseparatelyifyouneedtheinducibleexpression).
Thelentiviruscontainsa Blasticidin-RFP fusiondualMarkerundertheRSVpromoterwhichallowsfluorescencesortingorblasticidinkilling selectionof transducedcells. TheRFPsignalprovidesconvenient,real-timemonitoringofthevirus’ performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManual fordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP1059
