ThisPre-madelentivirusexpressesaCDNAclone, human target: SPARC (secretedproteinacidicandcysteinerich). (alternativename:BM-40;ON).ItisexpressedwiththeHAtagatitsC-terminal.Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID: NM_003118
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducIBLe suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction),oroptionallyusedfor inducibleexpression by tetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent(PremadeTetRlentivirus canbepurchasedseparatelyifyouneedtheinducibleexpression).
Thelentiviruscontainsa Blasticidin-RFP fusiondualMarkerundertheRSVpromoterwhichallowsfluorescencesortingorblasticidinkilling selectionof transducedcells. TheRFPsignalprovidesconvenient,real-timemonitoringofthevirus’ performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManual fordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP1057
