Pre-madeoptionalinducIBLelentiviralparticlesforexpressinghumantarget:SNCA(alternativename:ACP,PARK1,PARK4,PD1).Thesub-clonedcodonsequenceisidentical(100%match)toCDSregioninNCBIID:NM_000345.3.ParticlesalsocontainsaRFP-BlasticidindualselectionMarker.
Full sequenceverifiedtargetisnativelyexpressedunderatetracyclineinduciblesuCMVpromoter(Note:itcan alsousedasregularconstitutivehighexpressionwithoutanyinducationrequired).ARFP-BlasticidinfusionmarkerunderRSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibiotic(Dualmarkers).RFPsignalprovidesaconvenient,realtimemonitoringtheparticlesperformance.
Eachparticleswasvalidatedinlotbylotbasisandtargetexpressionisguaranteed.Particleswerepackagedinserum-freesolutioncontainingnoanyhumanoranimalorigincomponents.SeeProductManual fordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10xPolybrene.
Cat#:LVP507
