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Gentarget/h SMARCA1 inducible lentiviral particles/LVP614/1 Ea
ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,SMARCA1: SWI/SNFrelated,matrixassociated,actindependentregulatorofchromatin, (alternativename:ISWI;NURF140;SNF2L;SNF2L1;SNF2LB;SNF2LT;SWI;SWI2 ).Thesequenceis100%matchedCDSregion (NCBIID:NM_003069 ).
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpressionorinducibleexpressionwithtetracyclineorDoxwhenthetetracyclinerepressorprotein(TetR)ispresent.SeeProductManualfordetails.A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene.(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionforhigherprice).
Cat#:LVP614
