ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,SLC31A1:solutecarrierfamily31 (alternativename:COPT1;CTR1).Thesequenceis100%matchedCDSregion (NCBIID:NM_001859.3).
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpressionorinducibleexpressionwithtetracyclineorDoxwhenthetetracyclinerepressorprotein(TetR)ispresent.SeeProductManualfordetails.A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene.(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionforhigherprice).
Cat#:LVP602
