Pre-madeoptionalinducIBLelentiviralparticlesforexpressinghumantarget:hPTEN(alternativename:BZS;DEC;GLM2;MHAM;MMAC1;PTEN1;TEP1).Thesub-clonedcodonsequenceisidentical(100%match)toCDSregioninNCBIID:NM_000314.4.ParticlesalsocontainsaRFP-BlasticidindualselectionMarker.
Full sequenceverifiedtargetisnativelyexpressedunderatetracyclineoptionalinduciblesuCMVpromoter(Note:itcan usedasregularconstitutivehighexpressionwithoutanyinducation,andalsoastetracyclineinducibleexpressionwhen desired).ARFP-BlasticidinfusionmarkerunderRSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibiotic(Dualmarkers).RFPsignalprovidesaconvenient,realtimemonitoringtheparticlesperformance.
Eachparticleswasvalidatedinlotbylotbasisandtargetexpressionisguaranteed.Seeproductmanualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlinDMEMmedium.
Cat#:LVP534
