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Gentarget/h PDGFRB inducible lentiviral particles/LVP843/1 Ea
ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,PDGFRB (platelet-derivedgrowthfactorreceptor,betapolypeptide ) (alternativename:CD140B;IBGC4;IMF1;JTK12;KOGS;PDGFR;PDGFR-1;PDGFR1;PENTT). Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID:NM_002609.3 .
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction)orusedfor inducibleexpression withtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.
A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP843
