Pre-madeoptionalinducIBLelentiviralparticlesforexpressinghumantarget:hMCR4(alternativename:MC4-R).Thesub-clonedcodonsequenceisidentical(100%match)toCDSregioninNCBIID:NM_005912.2.
Full sequenceverifiedtargetisnativelyexpressedunderatetracyclineoptionalinduciblesuCMVpromoter(Note:itcan usedasregularconstitutivehighexpressionwithoutanyinducation,andalsoastetracyclineinducibleexpressionwhen desired).ARFP-BlasticidinfusionMarkerunderRSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibiotic(Dualmarkers).RFPsignalprovidesaconvenient,realtimemonitoringtheparticlesperformance.
Eachparticleswasvalidatedinlotbylotbasisandtargetexpressionisguaranteed.Seeproductmanualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlinDMEMmedium.
Cat#:LVP537