Pre-madeoptionalinducIBLelentiviralparticlesforexpressinghumantarget:KLF1(alternativename:EKLF,HBFQTL6,INLU).Thesub-clonedcodonsequenceisidentical(100%match)toCDSregioninNCBIID:NM_006563.3.ParticlesalsocontainsaRFP-BlasticidindualselectionMarker.
Full sequenceverifiedtargetisnativelyexpressedunderatetracyclineinduciblesuCMVpromoter(Note:itcan alsousedasregularconstitutivehighexpressionwithoutanyinducationrequired).ARFP-BlasticidinfusionmarkerunderRSVpromoter,allowstosortorselecttransducedcellsforlongtermexpressionviaRFPsignalorviablasticidinantibiotic(Dualmarkers).RFPsignalprovidesaconvenient,realtimemonitoringtheparticlesperformance.
Eachparticleswasvalidatedinlotbylotbasisandtargetexpressionisguaranteed.Particleswerepackagedinserum-freesolutioncontainingnoanyhumanoranimalorigincomponents.Seeproductmanualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlinserum-freemedium.
Cat#:LVP487
