ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,KIR3DL1 (killercellimmunoglobulinlikereceptor,threeIgdomainsandlongcytoplasmictail1) (alternativename:CD158E1;KIR;KIR3DL1/S1;NKAT-3;NKAT3; NKB1;NKB1B). Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID: NM_013289.2.
Thefull sequence-verifiedtargetisexpressedwithC-terminal6Histagunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction)orusedfor inducibleexpression withtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.
A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP1081
