ThisPre-madeoptionalinducIBLelentiviralparticles expresseshumantarget,KCNMB3: (potassiumlargeconductancecalcium-activatedchannel,subfamilyMbetamember3) (alternativename:BKBETA3;HBETA3;K(VCA)BETA-3;KCNMB2;KCNMBL;SLO-BETA-3;SLOBETA3 ). Thesub-clonedcodonsequenceisidentical(100%match)toCDSregion inNCBIID:NM_171830.
Thefull sequence-verifiedtargetisnativelyexpressedunderanoptionalinducible suCMVpromoter. Thislentiviruscan beusedforregularconstitutivehighexpression(withoutanyinduction)orusedfor inducibleexpression withtetracyclineorDoxinductionwhenthetetracyclinerepressorprotein(TetR)ispresent.
A Blasticidin-RFP fusiondualMarkerundertheRSVpromoterallowsfluorescencesortingorblasticidinresistanceselectionof transducedcells. TheRFPsignaladditionallyprovidesconvenient,real-timemonitoringoftheparticles’performance.
EachLentivirusisvalidatedonlot-by-lotbasis, andtargetexpressionisguaranteed. SeeProductManualfordetails(.pdf).
Amount:200ul,at1x107IFU/mlin DMEMmediumwith10%FBSand10xofpolybrene(60ug/ml).(Note:Whenspecificallyrequested,theconcentratedparticlescanbeprovided at1x108IFU/mlin200ulPBSsolutionfor$650.00USD).
Cat#:LVP732
